ELISpot Plus: Bovine TNF-α (HRP)

ELISpot Plus: Bovine TNF-α (HRP)

3012-4HPW-2

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Documents
Questions?
The easiest way to achieve reproducibility in ELISpot.

Components

Detection mAbMT771, biotin
Enzyme conjugateStreptavidin-HRP
SubstrateELISpot substrate: TMB for HRP
PlatePre-coated ELISpot plate, white MSIP (mAb MTF4918)

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Product specifications

Intended use

This ELISpot Plus kit is intended for the enumeration of cells secreting bovine TNF-α using the ELISpot assay. For research use only. Not for use in diagnostic procedures.

Serum/Plasma samples

Recommendation

Product details

ProductELISpot Plus: Bovine TNF-α (HRP)
ApplicationELISpot
AnalyteTNF-α
ReactivityCow
Specificity

This kit is based on a matched pair of mAbs specific for bovine (cow) TNF-α.

Shipping and Storage

Shipping

Shipped at ambient temperature.

Storage

Store antibodies, enzyme conjugate, and substrate at 4-8 °C upon receipt. Plates may be kept at room temperature.

Shelf lifeAt least 18 months from date of receipt.

PBMC from cow (100,000 cells/well) were incubated for 18 h in the absence or presence of LPS and the number of cells secreting TNF-α was analyzed by ELISpot.

Datasheet/Protocol
SDS

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TNF-α

Analyte description

Tumor necrosis factor (TNF), also known as TNF-α, is produced by many different cell types, e.g., monocytes, macrophages, T cells, and B cells. Among the many effects of TNF-α are protection against bacterial infection, cell growth modulation, immune system regulation, and involvement in septic shock.

Alternative namesTumor necrosis factor-α, TNF-α, TNF-alpha, TNF-a, TNFa, Tumor necrosis factor-alpha, TNF, TNF-a-alpha
Cell typeT cell, Tc, Th1, Th2, Th17, Tfh, Monocyte/MΦ
Gene ID280943